large klenow fragment new england biolabs cat (New England Biolabs)

New England Biolabs is a verified supplier

New England Biolabs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

New England Biolabs large klenow fragment new england biolabs cat
Large Klenow Fragment New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klenow+large+fragment/Klenow+Fragment/pm42800032-204-182-185
Average 99 stars, based on 7089 article reviews

large klenow fragment new england biolabs cat - by Bioz Stars, 2026-10

99/100 stars

Images

Labeling:

Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales.
Article Snippet: Chromosomal DNA was digested by adding 15 μL of 100 U/μL HindIII (NEB) and incubating for 4 h at 37°C. .. After heat inactivation of HindIII (65°C, 20 min), the HindIII DNA ends (50 μL) were filled and labeled with biotin by adding 10 μL of 0.4 mM biotin-14-dCTP (Thermo), 2 μL each of 2 mM dATP, dTTP, dGTP, 1.8 μL of 10 × NEBuffer 2, and 0.5 μL of 5 U/μL Klenow Large Fragment (NEB), with incubation at 20°C for 30 min. Additionally, for the sample of E233S cells, the chromosomal DNA was digested by Sau3AI (NEB). .. The digestion and fill-in reactions were quenched by adding 7.7 μL of 10% SDS and 1.4 μL of 0.5 M EDTA (pH 8.0) at 21°C for 5 min. After ligation at 16°C overnight using T4 DNA ligase (NEB), the samples were reversely cross-linked by adding 100 μL of 10% SDS, 50 μL of 0.5 M EDTA (pH 8.0), and 5 μL of 20 mg/mL proteinase K with incubation at 65°C for 6 h, AR TI CL E IN P RE SS followed by further incubation at 37°C for more than 6 h. The first DNA purification step was completed with a DNA Cycle-Pure Kit (Omega Bio-tek, Norcross, USA).

Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales
Article Snippet: Chromosomal DNA was digested by adding 15 μL of 100 U/μL HindIII (NEB) and incubating for 4 h at 37°C. .. After heat inactivation of HindIII (65°C, 20 min), the HindIII DNA ends (50 μL) were filled and labeled with biotin by adding 10 μL of 0.4 mM biotin-14-dCTP (Thermo), 2 μL each of 2 mM dATP, dTTP, dGTP, 1.8 μL of 10 × NEBuffer 2, and 0.5 μL of 5 U/μL Klenow Large Fragment (NEB), with incubation at 20°C for 30 min. Additionally, for the sample of E233S cells, the chromosomal DNA was digested by Sau3AI (NEB). .. The digestion and fill-in reactions were quenched by adding 7.7 μL of 10% SDS and 1.4 μL of 0.5 M EDTA (pH 8.0) at 21°C for 5 min. After ligation at 16°C overnight using T4 DNA ligase (NEB), the samples were reversely cross-linked by adding 100 μL of 10% SDS, 50 μL of 0.5 M EDTA (pH 8.0), and 5 μL of 20 mg/mL proteinase K with incubation at 65°C for 6 h, followed by further incubation at 37°C for more than 6 h. The first DNA purification step was completed with a DNA Cycle-Pure Kit (Omega Bio-tek, Norcross, USA).

Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales
Article Snippet: Chromosomal DNA was digested by adding 15 μL of 100 U/μL HindIII (NEB) and incubating for 4 h at 37 °C. .. After heat inactivation of HindIII (65 °C, 20 min), the HindIII DNA ends (50 μL) were filled and labeled with biotin by adding 10 μL of 0.4 mM biotin-14-dCTP (Thermo), 2 μL each of 2 mM dATP, dTTP, dGTP, 1.8 μL of 10 × NEBuffer 2, and 0.5 μL of 5 U/μL Klenow Large Fragment (NEB), with incubation at 20 °C for 30 min. Additionally, for the sample of E233S cells, the chromosomal DNA was digested by Sau3AI (NEB). .. The digestion and fill-in reactions were quenched by adding 7.7 μL of 10% SDS and 1.4 μL of 0.5 M EDTA (pH 8.0) at 21 °C for 5 min. After ligation at 16 °C overnight using T4 DNA ligase (NEB), the samples were reversely cross-linked by adding 100 μL of 10% SDS, 50 μL of 0.5 M EDTA (pH 8.0), and 5 μL of 20 mg/mL proteinase K with incubation at 65 °C for 6 h, followed by further incubation at 37 °C for more than 6 h. The first DNA purification step was completed with a DNA Cycle-Pure Kit (Omega Bio-tek, Norcross, USA).

Incubation:

Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales
Article Snippet: Chromosomal DNA was digested by adding 15 μL of 100 U/μL HindIII (NEB) and incubating for 4 h at 37 °C. .. After heat inactivation of HindIII (65 °C, 20 min), the HindIII DNA ends (50 μL) were filled and labeled with biotin by adding 10 μL of 0.4 mM biotin-14-dCTP (Thermo), 2 μL each of 2 mM dATP, dTTP, dGTP, 1.8 μL of 10 × NEBuffer 2, and 0.5 μL of 5 U/μL Klenow Large Fragment (NEB), with incubation at 20 °C for 30 min. Additionally, for the sample of E233S cells, the chromosomal DNA was digested by Sau3AI (NEB). .. The digestion and fill-in reactions were quenched by adding 7.7 μL of 10% SDS and 1.4 μL of 0.5 M EDTA (pH 8.0) at 21 °C for 5 min. After ligation at 16 °C overnight using T4 DNA ligase (NEB), the samples were reversely cross-linked by adding 100 μL of 10% SDS, 50 μL of 0.5 M EDTA (pH 8.0), and 5 μL of 20 mg/mL proteinase K with incubation at 65 °C for 6 h, followed by further incubation at 37 °C for more than 6 h. The first DNA purification step was completed with a DNA Cycle-Pure Kit (Omega Bio-tek, Norcross, USA).

Review

large klenow fragment new england biolabs cat (New England Biolabs)

Structured Review

Images

Related Articles

Labeling:

Incubation:

Similar Products

Image Search Results